17-Hydroxyprogesterone

Cleveland Heartlab #'s, H, Test

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NEW YORK DOH APPROVED: YES
CPT Code: 83498
Order Code: 17180
ABN Requirement: No
Synonym: Hydroxyprogesterone-17
Specimen: Serum or EDTA Plasma
Volume:  0.5 mL
Minimum Volume:  0.25 mL
Container: Serum Red-top (no gel), Plasma EDTA (lavender-top) or (royal blue-top), Sodium or Lithium heparin (green-top)

Collection:

Red Top Serum:

  1. Collect and label sample according to standard protocols.
  2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
  3. Allow blood to clot 30 minutes.
  4. Centrifuge 10 minutes.
  5. Aliquot serum into a labeled transport tube labeled as “Red Top Serum” and cap tightly.
  6. Store transport tube refrigerated at 2-8oC until ready to ship.

EDTA Plasma:

  1. Draw and gently invert 8 to 10 times.
  2. Centrifuge immediately for 10 minutes at room temperature.
  3. Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma layer.
    Note: This ensures that the buffy coat and red cells remain undisturbed.
  4. Aliquot plasma into labeled transport tube labeled as “EDTA plasma” and cap tightly. Discard original tube.
  5. Store transport tube refrigerated at 2-8oC until ready to ship.

Transport: Store serum or EDTA plasma at 2°C to 8°C after collection and ship the same day per packaging instructions provided with the Cleveland HeartLab shipping box.

Stability:

Ambient (15-25°C): 48 hours
Refrigerated (2-8°C): 7 days
Frozen (-20°C): 2 years

Causes for Rejection: Gross hemolysis, Serum separator tube (SST)

Methodology: Chromatography/Mass Spectrometry

Turn Around Time: 3-7 days

Reference Range:

Age/Stageng/dL
Adult Male18-30 Years32-307
31-40 Years42-196
41-50 Years33-195
51-60 Years37-129
Adult FemalePre-Menopausal Mid Follicular23-102
Pre-Menopausal Surge67-349
Pre-Menopausal Mid Luteal139-431
Postmenopausal Phase≤45
Pregnancy: First Trimester78-457
Pregnancy: Second Trimester90-357
Pregnancy: Third Trimester144-578
Pediatric1-11 Months≤147
1 Year≤139
2 Years≤134
3 Years≤131
4 Years≤131
5 Years≤133
6 Years≤137
7 Years≤145
8 Years≤154
9 Years≤166
10 Years≤180
11 Years≤196
12 Years≤213
13 Years≤233
14 Years≤254
15 Years19-276
16 Years23-300
17 Years26-325
InfantCord Blood1000-3000
Premature Infant (31-35 Weeks)≤405
Term Infant (12 hours)
Values decline gradually
to prepubertal levels		<460
<30 DaysNo Range Established
Tanner StagesII-III Male12-130
II-III Female18-220
IV-V Male51-190
IV-V Female36-200

Clinical Significance: 17-hydroxyprogesterone is elevated in patients with congenital adrenal hyperplasia (CAH). CAH is a group of autosomal recessive diseases characterized by a deficiency of cortisol and an excess of ACTH concentration. 17-hydroxyprogesterone is also useful in monitoring cortisol replacement therapy and in evaluating infertility and adrenal and ovarian neoplasms.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.