Hepatitis B Surface Antibody, Qualitative

CPT Code: 86706
Order Code: 499
Includes: Hepatitis B Surface Antibody, Qualitative
ABN Requirement: No
Specimen:
     Preferred: Serum
    Alternative: EDTA Plasma
Volume:  1.0 mL
Minimum Volume:  0.5 mL
Container:
Preferred: Gel-barrier Tube (SST)
     Alternative: EDTA (Lavender Top) Tube

Collection:

Serum:

1. Collect and label sample according to standard protocols.
2. Gently invert tube 5 times immediately after draw. DO NOT SHAKE.
3. Allow blood to clot 30 minutes.
4. Centrifuge for 10 minutes.

EDTA Plasma:

1. Collect and label sample according to standard protocols.
2. Gently invert tube 10 times immediately after draw. DO NOT SHAKE.
3. Centrifuge specimen for 10 minutes.
4. Pre-squeeze transfer pipet bulb and draw off approximately 2/3 of the upper plasma.
   Note: This ensures that the buffy coat and red cells remain undisturbed.
5. Aliquot plasma into transport tube labeled as “EDTA Plasma” and cap tightly. Discard original tube.

Transport: Store specimen at 2°C to 8°C after collection and ship the same day per packaging instructions included with the provided shipping box.

Stability:

Ambient (15-25°C): 5 days
Refrigerated (2-8°C): 14 days
Frozen (-20°C): 30 days

Causes for Rejection: Improper labeling; samples not stored properly; samples older than stability limits; gross hemolysis; gross lipemia

Methodology: Immunoassay (IA)

Turn Around Time:  1 to 3 days

Reference Range: Non-Reactive

Clinical Significance: The detection of anti-HBs is indicative of a prior immunologic exposure to the antigen or vaccine. To determine immune status as ≥10 mIU/mL as per CDC guidelines, please order Hepatitis B Surface Antibody, Quantitative.

The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed.